Video 2 . Pollen tube migration within an ovule in an in vitro guidance assay

Time-lapse images showing a GFP-tagged tube entering and navigating within the ovule.

The growth rate of the pollen tube decreases substantially before and soon after entry.

Within the ovule, pollen tube ceases growth presumably in one of the synergids and tube discharge occurs, leaving a visible GFP spot.

Ref: Palanivelu & Preuss (2006), BMC Plant Biology; 6:7.